According to a study published yesterday, Revisiting the harem conspiracy and death of Ramesses III: anthropological, forensic, radiological, and genetic study, Y- STR data places his YDNA haplogroup in E1b1a using Whit Athey's Haplogroup predictor:
"Genetic kinship analyses revealed identical haplotypes in both mummies (table 1⇓); using the Whit Athey’s haplogroup predictor, we determined the Y chromosomal haplogroup E1b1a. "
his DYS repeats are listed as follows:
| DYS 19 | 19 |
| DYS 385a,b | 20 |
| DYS 389I | 13 |
| DYS 389II | 33 |
| DYS 390 | 21 |
| DYS 391 | 8 |
| DYS 392 | 17 |
| DYS 393 | 8 |
| DYS 437 | 14 |
| DYS 438 | 10 |
| DYS 448 | 20 |
| DYS 456 | 13 |
| YGATAH4 | 13 |
Plugging these numbers in Whit Athey's predictor does indeed indicate that his haplogroup is E1b1a with 99.1% probability using equal priors. The decisive DYS, to judge between E1b1a and E1b1b, is DYS 390, with the exclusion of DYS 390, his haplotype belongs to 83.7 % E1b1b and 15.8% E1b1a, however, it is well known that DYS 390 = 21 is a high probability signature for West/Central/Southern Africa, i.e. where E1b1a dominates (see below).
UPDATE : Upon receiving an e-mail stating that the haplotype could still belong to E1b1b, on the basis of a haplotype from Chad present in the FTDNA database that has DYS 390 = 21, I further looked into it, the presence of such a haplotype would not necessarily refute what the authors of this study are claiming, because if one enters the repeats for those Chad E1b1b haplotypes (but only for the same DYS#'s that are included this study) into the predictor, i.e. :
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one would still get an assignment to haplogroup E1b1b with 88.6% probability and only a 5.6% probability that the haplotype may belong to E1b1a, therefore, I highly doubt that this pharaoh's haplotype, if extracted correctly and with out contamination, would be anything but E1b1a, absent an SNP test however, one can never be 100% sure.
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Please clarify your last paragraph saying "anything but e1b1a"
ReplyDeleteYou stated 88.6% likelihood of being e1b1b, so you are saying that you doubt it would be anything but e1b1b?
~ Mike Dammann
http://www.rhesusnegative.net
The communication I received implied that the predictor was simply just confusing Ramesses' E1b1b haplotype for an E1b1a haplotype due to DYS 390 being equal to 21 repeats, since in-fact, there are (rare as they maybe) cases of E1b1b haplotypes that also have DYS 390 = 21, the email pointed to the Chadian FTDNA haplotype as one exemplary sample.
DeleteI thought that was an interesting supposition, however, it was wrong, why? because when you enter those SNP tested Chadian Y-STR in the predictor, they are not incorrectly predicted as E1b1a, which would be the case we would expect if the Pharoah's haplotype were being genuinely confused, rather the Chadian haplotype was correctly being predicted as E1b1b. Is that any clearer ?
So is it more likely for Ramses the third to be e1b1a because days 390 - 21 is a key signiture for west/ central Africa while 390- 21 is rare for e1b1b
DeleteYes, thank you. So in other words: We have narrowed things down to two good guesses one of which would be certain.
ReplyDeleteAnother question: Are there other indicators you might be aware of which could lead me into the right direction?
I would like for you to confirm whether you have credible data on the following:
1) Claims that Ramses II was HLA-B27 positive.
2) That the cde frequency amongst mummie ramains tested for blood type was high (the socalled "rh negative gene")
I am getting claims thrown at me that Ramses II was supposedly B negative and would like for you to let me know what your take on this is.
Thank you in advance for your help.
~ Mike
Marianne Luban put the STRs of Ramses III into popaffiliator program and they presented as sub-Saharan, as well.
ReplyDeletehttp://thetimetravelerreststop.blogspot.fr/2012/12/here-is-what-came-out-when-i-put-dna-of.html
To further check the validity of popAffiliator (http://cracs.fc.up.pt/~nf/popaffiliator2/) using the same 9 STRs, I ran the STRs of a few samples from DNATribes website (http://www.dnatribes.com/sampleresults.html). I also used popAffiliator 2 as it is a liitle bit more accurate that popAffiliator 1.
As we can see below, ALL of them were assigned to their correct Continental groups (Sub-Saharan Africa, Eurasia, Asia).
Just note these are NOT admixture proportions but just probabilities. So it seems that even 8 markers are enough to assign the correct continental groups with Sub-Saharan individuals always getting a Sub-Saharan probability > 90%.
9 STRS used(CSF1PO,D2S1338, D3S1358,D13S317, D16S539, D18S51, D21S11, FGA, D7S820)
Ramses III:
Sub-Saharan Africa 96.2%
Eurasia 2%
Asia 1.8%
(D3S1358 was defaulted as mentionned by Marianne but using any value for D3S1358, Ramses III always gets a probablity > 90% for sub-Saharan Africa.)
1) Sub-saharan individuals
African America:
Sub-Saharan Africa 99.2%
Eurasia 0.6%
Asia 0.3%
Uganda:
Sub-Saharan Africa 93%
Eurasia 4.3%
Asia 2.7%
2) Eurasian individuals
South Italian:
Eurasia 59.2%
Asia 28.8%
Sub-Saharan Africa 12%
Berber:
Eurasia 47.4%
Sub-Saharan Africa 37%
Asia 15.6%
Saudi Arabian:
Eurasia 45.6%
Sub-Saharan Africa 42.7%
Asia 11.6%
Scottish:
Eurasia 63.4%
Sub-Saharan Africa 31%
Asia 5.6%
Turkish:
Eurasia 62.6%
Sub-Saharan Africa 19.6%
Asia 17.8%
3) Asian individuals
Korean:
Asia 77%
Eurasia 18.6%
Sub-Saharan Africa 4.4%
Interesting, for the sake of precision, the populations used to train the machine learning model to identify Subsahran Africans are 195 Namibians, 404 Somalis, 225 Tanzanians and 47 unspecified SSA.
DeleteThe publication for popaffiliator, Pereira et.al can be found here https://oda.hio.no/jspui/bitstream/10642/472/2/529937post.pdf
Another point is that there are 3 datasets that the publication specifies, one is the 3R, which encompasses West Asia, SSA and East Asia, apparently the dataset that your friend used above, however there are 2 more, the 5R which encompasses “Asia, Eurasia, Sub-Saharan Africa, North Africa and Near East”, and the 7R, which encompasses all the 7 regions in the database. I suspect using the other 2 datasets may yield some different results, note that the North African training populations includes, among others, Egyptians from Siwa and Southern Egypt, and possibly berbers from Morroco as well.
Those are False statistics and classifications.
DeleteDnaTribes just released a new study which seems to confirm "evidence of Sub-Saharan African ancestry (possibly among several ancestral components) for pharaonic families of ancient Egypt".
ReplyDelete"Digest for February 2013: Ramesses III and African Ancestry in the 20th Dynasty of New Kingdom Egypt" : http://www.dnatribes.com/dnatribes-digest-2013-02-01.pdf
18th Dynasty Population Affiliations http://thetimetravelerreststop.blogspot.fr/2012/12/18th-dynasty-predicted-ethnicity.html
ReplyDelete•"Genetic kinship analyses revealed identical haplotypes in both mummies (table 1⇓); using the Whit Athey’s haplogroup predictor, we determined the Y chromosomal haplogroup E1b1a. The testing of polymorphic autosomal microsatellite loci provided similar results in at least one allele of each marker (table 2⇓)."
--Hawass et al 2012. Revisiting the harem conspiracy and death of Ramesses III. British Medical Journal, BMJ2012;345:e8268
•Haplogroup E1b1a (now known as E-M2) is an
African DNA group, most commonly found in sub-Saharan Africa QUOTE:
"Haplogroup E1b1 now contains two basal branches, E-V38 (E1b1a) and E-M215 (E1b1b), with V38/V100 joining the two previously separated lineages E-M2 (former E1b1a) and E-M329 (former E1b1c). Each of these two lineages has a peculiar geographic distribution. E-M2 is the most common haplogroup in sub-Saharan Africa, with frequency peaks in western (about 80%) and central Africa (about 60%)."
--Trombetta et al 2011. A New Topology of the Human Y Chromosome Haplogroup E1b1 (E-P2)
PLoS ONE 6(1): e16073.
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•DNA Tribes Digest for January 1, 2012: Last of the Amarna Pharaohs: King Tut and His Relatives... http://www.dnatribes.com/dnatribes-digest-2012-01-01.pdf
•DNA Tribes Digest for February 1, 2013: Ramesses III and African Ancestry in the 20th Dynasty of New Kingdom Egypt... http://www.dnatribes.com/dnatribes-digest-2013-02-01.pdf
Meu DNA e y=E1b1a7a também pertence ao Faroe HAMSES |||
ReplyDeleteO que te faz dizer E1b1a7a especificamente? Não me lembro de nenhum sibclade sendo especificado.
DeleteThey are comfirmed E1b1a sorry racist! Even if he was E1b1b that doesn't mean he is white. 90% of whites belong to R1b l R1b has nothing to do with E. I have no idea where white people get of claiming E1b1b is white when most E1b1b carriers are Black. The only So-Called caucasians that Belong to E or eurocentrist favorite E1b1b is people with recent African ancestry. E1b1b is E1b1a younger brother. Both originated somewhere between Ethiopia and Iran. The so-called Bantu migration myth has been debuked. The real so-called bantu orginated in Ethiopia or Saudia Arabia then went further west to modern day cameroon. It's confirmed that Ethiohilex and Deneikos Pontikos are racist eurocentrist pseudoscentist.
ReplyDelete